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KMID : 0880220120500061014
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Journal of Microbiology
2012 Volume.50 No. 6 p.1014 ~ p.1023
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Screening of mutant strain Streptomyces mediolani sp. AC37 for (-)-8-O-methyltetrangomycin production enhancement
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Jakeline Trejos Jimenez
Maria Sturdikova
Vlasta Brezova
Emil Svajdlenka
Marta Novotova
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Abstract
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Streptomyces mediolani sp. AC37 was isolated from the root system of higher plant Taxus baccata and produced metabolite identified as (?)-8-O-methyltetrangomycin according to LC/MS/MS analysis. In our screening program for improvements of bioactive secondary metabolites from plant associate streptomycetes, mutation was used as a tool for the induction of genetic variations for selection of higher (?)-8-O-methyltetrangomycin producers of isolates. S. mediolani sp. AC37 was treated with UV irradiation and chemical mutagenic treatment (N-nitroso-N-methyl-urea). The radical scavenging and antioxidant capacity of (?)-8-O-methyltetrangomycin and extracts isolated from mutants were tested using EPR spin trapping technique and ABTS·+ assay. Comparison of electron microscopic images of Streptomyces sp. AC37 and mutant strains of Streptomyces sp. AC37 revealed substantial differences in morphology and ultrastructure.
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KEYWORD
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Streptomyces mediolani, (-)-8-O-methyltetrangomycin, mutation, N-nitroso-N-methyl-urea, EPR spectroscopy, sulphate radical anion, antioxidant
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